Hairy root culture of withania somnifera

(Indian ginseng) is an central medicinal works which yields pharmaceutically about compounds named withanolides. The present work deals with optimization of parameters of hirsute plant organ culture of for the production of biomass and withanolide A. We also investigated the effects of carbon informant [sucrose, glucose, fructose, maltose, glucose fructose (1:1), fructose sucrose (1:1) and disaccharide aldohexose (1:1)], sucrose concentration (1%, 2%, 3%, 4%, 6% and 8%) and the initial intermediate p H (4.0, 4.5, 5.0, 5.5, 5.8, 6.0 and 6.5) on growth and production of withanolide A in fuzzed root cultures of .

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Increased production of withanolide A, withanone, and withaferin A in hairy root cultures of Withania somnifera (L.) Dunal elicited with methyl jasmonate and salicylic acid | SpringerLink

An central medicinal plant that possesses a miscellany of bioactive secondary metabolites jointly legendary as withanolides. Hairy roots with an initial matter mass of 5 g FW were elicited singly with methyl group jasmonate (Me J) and salicylic acid (SA) at several concentrations for diametric exposure times aft 30 life of culture. Enhanced production of fuel (32.68 g FW and 5.54 g DW; 1.23-fold higher), withanolide A (132.44 mg/g DW; 58-fold higher), withanone (84.35 mg/g DW; 46-fold higher), and withaferin A (70.72 mg/g DW; 42-fold higher) were achieved from 40 day-old harvested hairy plant organ induced with 150 μM SA for 4 h exposure time.

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Expression of important pathway genes involved in withanolides biosynthesis in hairy root culture of Withania somnifera upon treatment with Gracilaria edulis and Sargassum wightii - ScienceDirect

(50%) in MS liquid radical average enabled maximum creation of dry biomass (5.46 g DW) and withanolides contents (withanolide A 5.23 mg/g DW; withaferin A 2.24 mg/g DW and withanone 4.83 mg/g DW) in hairy root word afterward 40 days of acculturation with 48 h contact time. The obtained withanolides table of contents were importantly higher (2.32-fold–2.66-fold) in comate root society when compared to the control. RT PCR analysis of consequential nerve pathway genes such as SE, SS, HMGR and FPPS exhibited essential graduate facial expression upon the alga extracts treatment in hairy root culture.

Synthesis of withanolide A depends on carbon source and medium pH in hairy root cultures of Withania somnifera - ScienceDirect


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